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Cryopreservation tube automatic production line-the use method and precautions of cryopreservation tube

Release Date:2022-10-15 11:35:07 Popularity:

  GST Technology's cryopreservation tube automatic production line can be used for automatic feeding, printing, drying, capping, testing, bagging, encapsulation and coding of lyophilised tubes. Learn more about GST Technology's lyophilisation tubes below!

Cryopreservation tube automatic production line-the use method and precautions of cryopreservation tube

  A lyophilisation tube, also known as a strain storage tube, is a laboratory container for strain storage or transfer.

  Steps for using lyophilisation tubes.

  1. Pick a fresh culture from a pure culture of bacteria and inoculate it with a suspension of approximately 3-4 maltosities in the strain lyophilisation tube.

  2. Tighten the lyophilisation tube and turn it back and forth 4-5 times to emulsify the bacteria without shaking.

  3. Store the lyophilisation tubes in the refrigerator (-20°C - 70°C).

Cryopreservation tube automatic production line-the use method and precautions of cryopreservation tube

  Recommendations for the safe use of lyophilisation tubes.

  1. When using lyophilisation tubes for storage of samples, it is strictly required that the tubes should be stored in the vapour phase layer of liquid nitrogen or in a refrigerator. If the tubes are stored in liquid nitrogen, there is a certain chance that the liquid nitrogen will seep inside the tubes and cause an imbalance in pressure between the inside and outside of the tubes due to vaporisation during resuscitation, which could easily cause the tubes to blow up and be biohazardous.

  2. It is recommended that you wear a lab coat, cotton gloves and operate on a safe laboratory bench. Wear goggles or a protective face shield if conditions permit. Please take extra care as the room temperature is higher in summer than in winter.

Cryopreservation tube automatic production line-the use method and precautions of cryopreservation tube

  3. During storage of frozen cells, the freezing temperature of the tubes must be uniform. Uneven freezing will result in the development of ice plugs, which will inhibit the transfer of fluid temperature on both sides, creating dangerously high pressures and causing damage to the tubes.

  4. Do not freeze more samples than the maximum working volume required for the tube.


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